Silver Staining for Spirochetes in Tissues: Rationale, Difficulties, and Troubleshooting
نویسندگان
چکیده
منابع مشابه
Spirochetes in Body Fluids and Tissues
In the 1960's, it was demonstrated that Treponema pallidum can persist in the lymph nodes of patients with penicillin-treated late syphilis, and that the organism can be found in aqueous humor and spinal fluid in cases of late seronegative lues as well. Research in this field obviously requires acceptable standard methods. Following a workshop on the subject held at the Center for Disease Contr...
متن کاملUtility of Silver Nitrate Staining in Detection of Spirochetes from Oral Cavity of Periodontally Healthy and Diseased Individuals
Background: Oral spirochetes play a definitive role in the etiology of periodontitis. But their study is hampered due to difficulty in cultivating them and molecular methods are expensive. Hence the study explores the possibility of using staining as a screening procedure for the study of oral spirochetes. Experimental approach: The study included 100 patients with chronic periodontitis and 100...
متن کاملSpirochete: Silver Staining for Nucleic Acids
Borrelia burgdorferi the Lyme disease spirochete was grown in modified Kelly medium and characterized by transmission and by scanning electron microscopy. Using silver staining procedures which preferentially bind to nuclear components of eukaryotic cells, signal could be detected by backscattered electron imaging throughout the length of the prokaryotic spirochete. Interestingly, however, the ...
متن کاملpassivity in waiting for godot and endgame: a psychoanalytic reading
this study intends to investigate samuel beckett’s waiting for godot and endgame under the lacanian psychoanalysis. it begins by explaining the most important concepts of lacanian psychoanalysis. the beckettian characters are studied regarding their state of unconscious, and not the state of consciousness as is common in most beckett studies. according to lacan, language plays the sole role in ...
Silver Staining of Alzheimer's Disease.
Paraffin sections were deparaffinized and hydrated to distilled water, then placed in 20% silver nitrate and kept in 60°C for 15 minutes, then rinsed in distilled water. Then sections were treated with formalin solution containing ammoniacal silver solution for about 15 min, followed by rinsing and placing tissue in sodium thiosulfate solution for 2 minutes and washing in tap water. The section...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Laboratory Medicine
سال: 2002
ISSN: 0007-5027,1943-7730
DOI: 10.1093/labmed/33.9.705